>microbiology lab >culture/ slide comes out really well

>>9164666
lick it. give the bacteria a second chance

Depending on the stain just take it with you, everything is dead, can't hurt anyone outside of the lab.

>When you give the cells your plasmid with antibiotic resistance, letting the smart ones take up your plasmid while your murder the stupid little cells that defied your will
>When you control the extent to which the cells grow
>When you induce and they all produce protein for you exactly the way you want
>tfw you're a god over tiny creatures

>>9164936
>tfw you are a god over tiny creatures

Isn't that why we took up bioscience, to play God?

>>9164936
That sound cool, it's like a simulation game but better.

>>9164936
>you're a god over tiny creatures
so you've become something that doesnt even exist and that they made up because they dont know it better, bravo anon

>making streak plate in bio lab with loop
>dumbass lab partner tries to cool the loop by touching it to the lid
>lid melts

File: 1398408530737.jpg (55KB, 500x806px) Image search: [Google]

55KB, 500x806px

>>9164666
>TAing microbiology lab
>teaching two-tube transfers
>pre-meds absolutely shitting the bed because this is probably the first time they've touched lab equipment before
>one girl comes in late during mandatory quiz
>the lab is quiet and yet she's like half-screaming in my ear about how she's sorry she's late
>"its okay just take a seat"
>"OKAY"
>what the fuck
>i check back with her in like half an hour
>undergrad TA is trying to help her but the sperg is being rude to everyone/everything in her vicinity
>partner looks at me and has a look on his face that says "kill me"
>imsorry.jpg
>girl attempts to do a transfer
>undergrad TA: "okay all you gotta do is stick the loop in and take it out slowly..."
>girl panics, takes the loop out too quickly, accidentally sprays broth on TA's face
>holy shit
>instantly remember the e coli strand is attenuated
>even then it's the principle: holy shit
>we later had a meeting after lab just talking solely about how much of a hazard this girl is

i like lab :^)

>>9166965
was she hot
did you fug

>>9167041
no

and no

>tfw C. Elegans RNA isolations are accurate and precise to a within a range of .02 ng/ul
Feels fuckin good mang

>practicing serial dilutions in 96 well plates
>get to the 4th well
>accidentally pipette the air right above the liquid before the tip gets submerged
>bubble inside the tip
>panic and expel the liquid back into the well
>shit ton of bubbles
>attempt to pop the bubbles by pipetting up and down again
>more bubbles

why do i suck at pipetting bros

>>9167099
Cause your inexperienced.
Took me 3 months in a research lab to get good

>>9167106
alright i can do this, i fucking got this next time

>>9167094
>Needing a whole roundworm to get your RNA
>Not just alcohol precipitating

>>9167099
Aside from getting gud, why is there so much diluent in your buffer?

>>9167138
is 50 microliters of diluent too much

>>9167138
>diluent
My bad my brain got scrambled I meant detergent.

>>9167144
kek

>>9167146
yeeee anyway if bubbles are a problem you can just make your buffer with less detergent. My go to is 0.05% tween by volume. You don't need a lot unless the stuff you're working with really likes to clump. If nonspecific binding is an issue I prefer to add BSA rather than more detergent. Detergent generally is a pain in the ass.

>>9167154
nah i feel you, it only happens like once in a blue moon and it pisses me off because i know it's due to shitty pipetting so i pretty much have to slow down and not be a retard

but i'll take your advice into consideration

I want to get into microbiology. How is it? Just ordered a book on algal cultures.

>tfw your Gram stains always come out as shit

>>9167167
Git gud at genetics, -omics in general, and bioinformatics if you want to really study it. Lots of indirect observation.

>>9164941
I'm not playing any more.

>>9167726
>new ip
you never were