Does ammonium uncouple photophosphorylation and by what mechanism? I'm seeing a lot of contradictory sources on this, especially the required concentration.
>>8561477
Yes, but a poor one.
It dissipates the proton gradient by converting it to heat.
Don't remember exactly how it works, but it's one of the following:
-blocks some ETC component, therefore no proton transfer across membrane
-binds to a channel, which opens and allows protons to pass through with lower delta G than ATP synthase, dissipating the gradient
Doesn't ammonia just diffuse through the inner membrane and then cause uncoupling through close enough dissociation constant, like Dinitrophenol?
Small shit gets through lipid bilayers and can transport protons that way.
>>8562606
Basically this, molecules like ammonium and dinitrophenol have an unequal distribution of electrons and therefore are able to disrupt the proton gradient and cause the protons to become heat rather than energy
>>8562606
>>8562876
Both the protonated and unprotonated forms of dinitrophenol penetrate membranes but they appear to have little to no effect on thylakoids according to https://www.ncbi.nlm.nih.gov/pubmed/13795285
On the other hand apparently only NH3 is permeable while the NH4 ion is not - https://www.ncbi.nlm.nih.gov/pubmed/5806578, https://www.ncbi.nlm.nih.gov/pubmed/16806203 - though I've heard the exact opposite claimed in http://www.sciencedirect.com/science/article/pii/S0176161704702667
Ideas?
>>8561477
Who is this beautiful woman?
>>8564186
That's Princess Caroline of Great Britain from the early 1800s, back when Apple still made decent phones like the one in the picture.