You can only post in this thread if you are refluxing a

>>8553475
I'm hitting the bong right now too but mine works a little different I think.

>>8553475
im refluxing a rxn in muh dick

>>8553483
>>8553486

HOLY FUCK WHAT DID I SAY

OUT

I transplanted mutant plant lines I'm going to take samples from for high throughout sequencing.

sorta like the miller-urey thing

ayy lmao

>>8553786
What's the function/purpose of the mutants? Or was it random mutation?

>>8553810
They're from a mutagenesis screen I'm performing. Looking for genes responsible for observed hyposensitivity to our signaling molecule after mutation.

>>8553813
how did you mutate them?

>>8553813
Righto.
>our signalling molecule
So you're comparing plant cells to mammalian cells? Or did I read that wrong? If not, why not just test on mammalian?

>>8553815
EMS. It causes random point mutations. You soak the seeds in a dilute solution for eight hours and then grow them up.

>>8553818
Sorry, could have worded that better. "The signaling molecule we study." And no, we're just studying plants. Plants have hormones too, anon.

>>8553821
what kind of pathway are you looking at (ex. auxin and development)?

>>8553821
>Plants have hormones too
Yeah I know, sorry I just didn't understand what you were doing. Seems interesting. Is random mutations better for your study than using a more specific gene of interest if you understand the pathways?

>>8553825
Forgive me but i don't want to say; if I specified the hormone there's a good chance that would be identifiable information given what I've said and I don't want to uncloak

>>8553838
Sorry for snarking, then. Yeah, it's a fishing trip more than anything. Just kind of looking for any accessory proteins that haven't been identified yet.

>>8553839
ok

>>8553841
>Just kind of looking for any accessory proteins that haven't been identified yet.
Alright cool.