Help! >PhD in bioengineering >trying to vortex proteins

Try different temperatures :^)

Try it at various pHs

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>>41405984

>PhD in bioengineering
>Ask advice on /fit/

choose one

>>41405984

Jizz on it. protein bitches love the sentient bipedal ape cock.

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>>41405984
stemcuck
!

ahahahaha!

>>41405984
Stealing this shit for my essay

>>41406091
Also ensure synthetic pathway doesn't involve any denaturants

>>41405984
Yeah man just put them on a nucelofusion program with a Krell's index of 1.7 and at an appropriate phase displacement. Make sure to keep it at a constant 700K, and leave it overnight, it should make the synthesis strong enough to keep integrity at vortexing of up to 8.95°. Good luck and let me know how it goes.

>>41405984
An accountant, a physicist, and a bioengineer each get pulled over by a cop at some point in the day. The accountant gets out of it by saying he will do the cop's taxes for free, along with saving him money. The physicist gets out of it by telling the officer how its impossible he was speeding according to the laws of physics and why the officer is mistaken. The bioengineer gets out of it, by getting on his knees and sucking the cops cock.

>>41405984
Depending on your exact set up you could still make this work by normalizing the signal. This feels like a badly done butthurt /sci/ troll after that thread the other month tho.

>>41405984
Try something other than vortex. I'm sure you could column it.

>>41406208
ayy lmao

>>41406237
Columns are slow af, this is high throughput shit.

>>41406213
A chemist, a biologist and an engineer are stuck on a remote island. The chemist gathers as many materials that he can to concoct chemicals that can be used for signalling for help and other practical purposes. The biologist begins cataloging biodiversity on the island, whats okay to eat and what has healing properties. The engineer just sucks cock.

>>41406258
>>41406237
yeah columns are really slow; dialysis is really slow too...

Centrifuging is the best but beads always clump and its a huge problem. I can't separate the clumps by pipetting fml

Maybe I should just use magnetic beads? has anyone had experience with that? Will they just pool together under a field and be separate or will they clump too once they make contact?

>>41405984
That seems more like an Overall Chromatograpy problem.
Have you considered a hplc?

>>41405984
Try lowering the temperature

>>41406288
I have never used magnetic beads but they seem to be used all the time in this sort of thing.

I was going to take a class in this sort of shit over the summer but hey ho I have a health issue so dunno bro.

>>41405984
how many scoops are you using currently?

>>41406399
Also something is niggling me at the back of my mind about the clumping. I used to know a fair bit on water engineering prior to biology so I'm not sure why it seems a familiar problem, but I'll post again if I remember anything decent.

>>41405984

i can count to potato

>>41405984
Something, something, PROTEIN, something, STRONGER... something something FORCE... something, something, complete shit.
I know a few of these words... try squats.

>>41405984
try using an Elisa plate its much easier for stuff like this

>>41406156
Fucking Mexican always steeling shit

>>41405984
>biology
>hard science

>>41405984
muh protein vortex has spiraled out of comtrol

>>41407767
wet science stamp collector

assuming the magnetism of the beads is important, could you use more magnetic beads? Or put your centrifuge/vortex/whatever into a strong magnetic field?

>>41405984

what did you call me

>>41406288
As for magnetic beads, that will depend on their size and coating. Nanoparticles just above the single domain size will pull to a magnet but so long as they're properly coated (with something like PEG) they should redipserse after. They have to be fairly small though (>200nm) otherwise the magnetism is too strong and they'll stay clumped even with a soluble coating.

I'm not sure if this exactly applies to what you're doing but magnetic nanoparticles are often used for their adsorption capabilities and ease of retrieval.

>>41407939
meant <200nm