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Anyone here who is studying medicine? I am working on what would

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Anyone here who is studying medicine?

I am working on what would closest be described as light therapy (or modulated light thereby)

Since i cannot morally justify experimenting on another human being all experiments are being done on myself

I am writing down the results and also keep record on my diet and mood and the time and season and radiation and wavelenght levels and

The experiments are with varying results, at one particular point i targeted the throat, and i suspect i hit the thyroid gland because the two following weeks i lost almost all body hair and a lot of weight due to loss of appetite, so i am more careful since then.

however lately ive grown impatient and i cranked up the intensity and i notice sporadic pains directly after treatment

the heart also feels like its working over time but its a pleasant feeling

does anyone care to evaluate these two last symptoms? with intense sporadic pains and intense heart beats? could i actually be coagulating blood inside me or is that ridiculous to suggest?
>>
>>719070303
Are you like, just blasting yourself randomly with a laser? Maybe you should do that under a doctor's supervision, you know, as a part of some official research.
>>
>>719070538

I use both lasers and LEDs as i am also taking the wavelengths in as a factor, i am staying away from the UV realm as what i researched points to that its nothing but harmful, and as i wrote before, it do not random blast myself, i do it only on one part of my body at a time and i keep the other side as a "control group" unless i want to trigger something that requires specific targeting
>>
>>719070538

But this is all background, all i with to know is a plausible explanation why the sudden pains right after treatment and the raise of heart rate
>>
>>719070303
You don't have to be doctor to say this:
You are a fucking moron.

Light is nothing but photons traveling with wavelengets at the visible spectrum.

Visible rarely has the energy to do anything on it's own before burning your retinas.

The use of lasers (high energy light) and UV frequencies can cause burns and destruction. Sunburns are superficial while radiation poisoning comes from intense rays of higher energy spectra.
These are already used in medicine to remove tumors.

At the same time, consistent UV helps with mood and Vitamin D production.

Nothing you can buy for under $10k will have any significant effect on your body beyond a tanning bed.
>>
>>719071497

my budget is well above $100000 so far, i am getting constantly complimented for my agility and youthful appearance although i am close to 40

Fuck you! as i stated before my explanation of what im doing is simplified, and i did not come here for advice on my research

I would like a reason why the raise of heart rate as and sudden pains as i cannot find info on that anywhere
>>
"research" lol
>>
I do medical research for a living, and nothing you've said so far indicates you have any experience with science beyond a generic high-school lab.

Please stop before you embarrass yourself further.
>>
>>719073653

Just fuck you

The company i am working for is currently funding heliospectra

Ive also collected medical studies that implicates that specific modulation not only faster tissue regeneration but fucking regrowth

so yeah i am trying to fine tune this, and yes i call it research.
>>
>>719073999

Im not afraid to embarrass myself on /b/, who would be? this is why i pose a simple question such as this one here, i expected to get a quick answer

sometimes i do get it sometimes i dont

i guess this is one of those times
>>
Clinical neurophysiologist here.

OP is a dumbass, Helio/phototherapy is bullshit unless you are talking about skin diseases like acne or psoriasis and shit.

OP, what you are experiencing is a placebo effect, stop that unless you want to develop skin cancer you fucking idiot.
>>
>>719074455
I'm sure you'll make more progress when you finally get into a 2-year tech school and take some gen-ed science classes.

Until then, keep shitposting on a Mongolian throat-singing forum about how awesome you are at using google.
>>
post pics of equipment + timestamp you lying fuck
>>
>>719074840
OP here

Clinical neurophysiologist

And what would a Neurophysiologist know about anything else than just the functionality of the nervous system?

are you a dermatologist as well?

i am calling your bullshit
>>
>>719070303
Try using radio waves and microwaves tuned to the frequency of viruses and cancer cells in order to eliminate them while leaving cellular tissue intact.

Try using harmonic integers withing the electromagnetic spectrum in order to facilitate healing.

Electromagnetic energy has healing potential especially when used with plant and herb extracts and mineral compounds.

Any thing in particular you are wondering about?
>>
>>719071059
If you knew the first thing about light then you would know it isn't going to penetrate the skin.

Near infrared wavelengths can penetrate tissue somewhat, but the very fact they penetrate so well also means they aren't absorbed by much and don't interact with much.

I'm a biochemistry fag who did my PhD dissertation on fluorescent proteins, so I know a thing or two about light and biology.
>>
>>719074676
You don't have proper controls. You know that you are shining the light. Your symptoms could just as well be due to, as >>719074840 suggested, a placebo effect.

It's clear you don't really how to design a proper experiment to remove bias. You are doing what is essentially a 5th grade-level science experiment.
>>
>>719070303
Did you know Viagra has been linked to melanoma?

Tell others, I am glad I have never tried it.
>>
>>719075616
Not op, but radiowaves can.

They are part of the electromagnetic spectrum along with visible light.
>>
Its bullshit OP.
Its the kind of shit you convince pet owners to get for their sick dogs because they dont know any better.
>>
>>719075353

this is exactly what im reading up on

however im looking for something that supposedly should trigger regrowth (inner hair cells has been clinically regenerated)

this paper was later removed and i only remember the wavelength spectrum and that it was modulated within the 10-500 khz area of modulation

but its not what im asking right now

i am experience sudden pain (can be anywhere) and an increase of hearth rate a few hours after treatment

id would like to know the origin
>>
>>719075621
OP is proposing a multivariable experiment with a n of 1, with no states timepoints, controls, or specific targets in mind?

If you were in my lab, I'd have fired you long ago. Your science is bad, you methodology non-existent, and any funding you would steal from other researchers would literally be putting us back as a species.
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>>719075297
Enough to know about alternative therapies such as that bullshit.
Also, it's basic celular function, if you give them too much radiation they flip and form neoplasms (and if you are unlucky, cancer).
If you give them a radiation too low it just... doesn't do anything really, maybe stimulate the production of vitamin D and whatnot.
All the current uses of Heliotherapy are for skin conditions, and that is all it will ever be, since you can't stimulate hepatic or neural regrowth with fucking light waves, not to lack of reasearch but due simply because that's not how cells work.

If you want to do "research", at least find someone with a skin condition before you give yourself thyroid cancer.
>>
>>719070303
your reading this your parents will die within 5 years to break the curse you must copy this to 5 pictures good luck. If you love your mom post this to 20 picture. One girl ignored this and her mom died 365 days later. Sorry I can't ignore this because I love my mom
>>
>>719075820
No they cant you ignorant shitlord.
>>
>>719070303
>i am a fucking retard that thinks infrequent exposure to different wavelengths of light are actually affecting me

Kill yourself.
>>
>>719075820
True, but radio also interacts with biological tissue very little. Part of my PhD work was solving the molecular structure of mutant variants of fluorescent proteins using nuclear magnetic resonance. Using extreme magnetic fields and radio waves to vibrate proteins to determine their structure. But even then special isotopes of carbon, hydrogen, nitrogen, and oxygen must be used to get the proteins to interact with the radio.

I had to use what is called "minimal media" to grow ecoli that had DNA of the protein of interest inside them. The minimal media contained nothing but salts and simple sugars of the special isotopes needed for NMR. Since those were the only materials present the ecoli grows and makes my protein of interest out of those special isotopes.

To get proteins to have strong interactions with radio you need to make them out of isotopes that are a tiny fraction of a percent of the natural ones in the real world.
>>
>>719075616

bullshit! it depends on the wavelength, and as i said before, i am working far away from the UV realm

florescent proteins huh? yeah i know what those are, and you probably use out dated camera equipment with your microscope because then resolution would be infinite

shut up, dont think i havent set fot in a lab before
>>
>>719076018
Cellular damage could be the issue if you are using frequencies which are tuned to healthy cellular tissue.

You need to do tests on isolated test samples in order to determine which ones are effective.

Don't kill yourself.
>>
>>719072262
Placebo
>>
>>719076268
Yes, radio waves can penetrate far trough solid objects.

If this were not true you could not receive radio transmissions indoors.
>>
>>719076467
>bullshit! it depends on the wavelength

That's exactly what I was saying, einstein... I say the wavelength matters and say you need to use near infrared wavelengths to penetrate tissue with light, and you come back saying the wavelength matters?

And I didn't use a microscope either. I used spectrometers, nuclear magnetic resonance, and x-ray crystallography. You clearly have no idea what you are talking about.

I honestly don't think you have any real scientific education or experience. You don't appear to be working with any controls and don't have any true experimental design to your "research"

You sound like some kid with a magnifying glass burning ants in his back yard.
>>
>>719076018
>i am experience sudden pain (can be anywhere) and an increase of hearth rate a few hours after treatment
This can be many things, but I'll give it a serious go:
1) Simple one, placebo. You expect a reaction, you get a reaction. The brain is a very powerful fucker, you'd be amased at how far the placebo effect can go.
2) It has something to do with your "experiments" ritual: Do you consume large amounts of coffee around that time? Do you do exercises or positions that put stress on your body?
3) Unrelated medical condition, possibly caused by malnutrition wich triggers muscle spasms.
4) And one of the more likely, fatigue, wich could cause all those symptons. Mixed with malnutrion and it's a bingo.
>>
How much time are you under those lights?
>>
>>719076467
Your experiment will not show anything that anyone will take seriously, because you have no control, the smallest sample size possible, bias due to knowing you are receiving the "therapy."

Do you even have a hypothesis as to what you believe will happen? One that is testable and falsifiable in a concrete way?
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>>719076396
Oscillating low amplitude Masers could direct the energy to the effected area allowing for the destruction of viruses and cancers.
>>
>>719076506

i document the intensity and wavelength + modulation pattern for every session, and i keep the space between treatment 1 months exactly

its hard to but be biased when you get such effects as losing all body hair, but i take it as a sign i stumbled on something

if not tissue regeneration then perhaps hormone control

but tissue damage could perhaps be an explanation, but i wonder why it shows up at other parts than those i treated?
>>
>>719077174
Sounds neat, now show the evidence.

>I'm working on a warp drive that can go faster than the speed of light. Right now it is only a sketch on a napkin, but you have no right saying it isn't possible... I'm a scientist.
>>
>>719076978
Not OP, but your research seems interesting. Did you have trouble getting the protein in question to crystallize well enough to do X-Ray crystallography on it? Did you manage to get the structure of the entire protein in question?
>>
Also, OP, you might have diabetes.
I suggest paying more attention to the smell of your piss and the amount of sugar you intake per day.
Could also be related to a fast metabolism.
I'd still seriously consider placebo though.
>>
>>719077220
Umm...

Stop what you are doing before you die.

You are killing yourself (if you are not trolling)

http://www.mayoclinic.org/diseases-conditions/radiation-sickness/basics/symptoms/CON-20022901
>>
>>719077220
>but tissue damage could perhaps be an explanation, but i wonder why it shows up at other parts than those i treated?

Which you will not know for sure because you don't have a proper control, you dolt.
>>
>>719077061

1. I could have a medical condition that i do not know of

2. fatigue could also be a plausible reason because i am illiterately working two jobs here

3. i have taken the possibility of delusion into account, the pain occurs a few hours after treatment and its quick but a little too intense to be my imagination
>>
>>719070303
You're studying medicine, presumably at an actual college or university, you're experimenting on yourself, and you're asking /b/ for help? Not, idunno, fellow med students?
>>
>>719070303
>Hit the thyroid gland- lost almost all hair and/or body weight

Doesn't work that way kiddo
>>
your study:
N=1
no science there. sorry.
>>
>>719076268
Omg....why did you decide to participate in this thread
>>
>>719077392
Evidence?

I gave you an idea based on what you said you were working on.

I thought you wanted to effect cells more precisely, a maser could* work.
>>
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>>719077466
Getting protein crystals is a black art. It's mostly trial and error. You set up a grid of test conditions in tiny wells in a plastic plate. Then you vary chemical conditions across the two axis.

For instance, on one plate you might vary the salt concentration in the y axis and the Ph in the X axis. That way you get a spread of many possible conditions across the plate. Then you spend hours under the microscope hunting for tiny crystals.

It's incredibly mind numbing and tedious. Then after weeks of this you finally get a crystal and it cracks and falls apart when you try to collect it for testing. Makes you just about go on a rampage and kill everybody in the building.
>>
>>719077988
> n=1
Another idiot shitposting
>>
>>719077793

Im good with electronics and software

reason i got into this was because i was sure i would get the modulation right

i am not a MED studient

med students are an obscure bunch in my country, not like yours
>>
>>719070303
wow
just WOW

i am a physicist and you just made me say out loud "wow".
i suggest you go to a doctor as soon as possible for those symptoms and right after you take care of that go see a therapists and discuss with him how you seem to imagine new physics laws
>>
>>719078117
Which is why Ph.Ds take so long, the story is ubiquitous. I get your pain, brosef.

Explains why Ph.D candidates drink so much. That is fascinating work, though. If you got good data, well done!
>>
>>719077951

i had throat ache and its the place i targeted for that particular session, i lost all hair and a lot of weight, i looked for the cause, and that was the most plausible explanation i could find.

but perhaps you know something i dont

all body hair gone (including genitalia) and weight loss

please explain... go...
>>
>>719078234
med students aren't scientists. Scientists experiment and try new things. Doctors atrictly adhere to established practices and procedures. Yes there are medical scientists, but they experiment in special settings using patients that know they are undergoing experimental procedures. Your average physician will get sued and lose their license if they "experiment" on anybody.

Doctors and scientists have very different minds.
>>
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>>719078234
>i am not a MED studient

I would have never guessed...
>>
>>719078445

OP here

still waiting smart asses...
>>
>>719078439
I was able to get structures and some data, enough to get a PhD, but I was not able to tell the entire story. It's a project that will take longer than the time it takes to get a PhD.

The ultimate goal of my project was to make a florescent protein that can be modified to bind a molecule of choice. Binding that molecule makes the florescence turn on/off. It can then become a real time indicator of the presence of that molecule.

We wanted to make one that could bind serotonin in neurons and in the synapses between neurons so it could show the neurons fire in real time. But I didn't get that far. I was able to get it to get brighter and darker in the presence of some molecules, but never turn off all the way.
>>
>>719078771
You want us to give you an explanation as to why YOUR experiment had the results it did? That is not our job, Mr. Scientist.

How about take an educated guess? What do YOU think happened?
>>
>cranked up the intensity and i notice sporadic pains
Internal hemorrhage
>intense heart beats
Explains above
>pleasant feeling
No idea probably fucked up your nerves
>>
>>719077220
Upload this into a wiki. The world needs to know your results.
Upload all the variables and methodology.

The phantom pains could be due to an effect of the light on the neural network. Along other 10000 things due to individual variability.

Assuming OP is not a full faggot, has access to equipment, etc...I have a question:

What was the wavelength, duration, intensity, etc for the experiment that caused most of your body hair to fall out? I would also assume you document this using photography. Post before and after pics plz
>>
>>719078984

The hair loss was unexpected, so i looked back if i could have hit something

the sport for that particular treatment was the throat, where the thyroid gland is located, it does hormone control

there it is...

but im not after hair loss i am after regenerating
>>
>>719079282
You are either a troll or a complete fucking retard
>>
>>719078445
First of all if you suspect that it's a thyroid disfunction and you really are a med student you would know tgat hair loss (hypothyroidism) and weight loss are (hyperthyroidism) contradict themselves and are mutuality exclusice...

What ur talikng about is large scake cytotoxicity like in chemotherapy or radiotherapy .. but there is nothing in the neck so it cant be caused by topical aplication of light.... i
At least not if its not severe rad poisoning. ..which woukd have caused at least lesions on the place of administration

As always OP IS A FAGGOT
>>
>>719078881
I can see the applicability of that protein, but it sounds like the protein would either have to be incredibly bright or the detection machinery would need to be very sensitive to distinguish from the background. I don't imagine serotonin would be in very high concentrations.

But hey, at least you tried, and you got something from it.
>>
>>719079148

internal hemorrhage and heartbeats are connected? AWESOME!!!

thank you! I was thinking something like that, but i wasnt sure

it only took a thread full of nonsense and a lot of insults to get here

perhaps should use less powers from now on

but i am also getting some pain resistance out of this, and i kinda like that...
>>
>>719079282
Look at it as two sides of the coin.

Post pics of your hairless dick
>>
>>719079771
So, your end conclusion is that you should lower the dosage, not, you know, stop before more damage occurs?
>>
>>719079690
Green fluorescent protein variants are incredibly bright. Some of the brightest known to man.

Here is a cool picture somebody bade using bacteria on a petri dish. The different colors are different cell lines of bacteria genetically modified to grow different variants of the fluorescent proteins.

I worked with the deepest red and near infrared variants because red light penetrates tissue the best.
>>
>>719079957
come on, do you really buy this shit
>>
I'm not a scientist but I can give you one useful piece of advice, don't come to /b/ with a question like that. Even if you find someone with enough scientific knowledge to help you, they'll probably use it to try and trick you into doing something that will cause permanent damage.
>>
>>719079771
Not sure if trolling but if you think that those sympots are nothing to be concerned of then you will most likely fry your brain in the end.
>>
>>719080030
Lol, I know GFPs are bright, i work with them as well. They are useful for studies involving protein localizations, 9/10 would recommend GFPs.

And you are indeed correct, higher wavelength lights are useful for thick tissues because they penetrate relatively well.

I guess I answered my own question. Maybe GFPs can be used to detect serotonin at a level above background. More research on my part is needed.
>>
>>719079185

Phantom pains could be as intense as real ones, and this is due to damaged nerves? that i didnt think of that, i really didnt.

and the raise of heart beats could be of pain induced stress i guess

the targeting the throat did make all hair fall out, but im not doing that again anytime soon.

im not going to tell the specific modulation or wavelength but it is within the red/infrared region

i dont know a good way to put intensity but i could put it as 180j/cm2

thats the most energy i ever absorb
>>
>>719079957

yes i guess, i am satisfied with that conclusion
>>
>>719080237

i am not new to be, but it is helpful sometimes, there are a lot of talented people here (believe it or not)

if you want to know how to store data for a long time, you get a lot of shit until someone tells you M-disk

its the same thing all the time, i am close to 40 if you didnt know
>>
>>719079185
>>719079821

not going to post pic without properly knowing how to remove exif
>>
>>719070303
One of the most causes of the instense heartbeats is medical conditions in your case it should be due to thyroid disease since you fucked with it. Also, hormonal changes which occured also explains it. And yes i study medicine and this is some basic knowledge you should know.
>>
>>719080640
this idiot wouldn't even share a wavelength cause he knows we're not dumb enough to fall for his stupid shit
>>
>>719080595
The tricky part is mutating the protein to have a binding pocket that can bind another molecule and to have the conformational change between bound and unbound states cause the flourophore to go inactive.

The flouophore is not a normal amino acid but modified ones that react with each other after the protein is made to create a fluorescent molecule, this can take hours. So to have it turn on and off instantly it needs to be formed prior to the on/off test.

Like I said, much more than what can be accomplished in a single PhD.
>>
>>719079630

I am NOT a med student and that was still my own conclusion

and i DO lesions from time to time

but then i just shift treatment to the previous and they disappear
>>
>>719080733
Feel free to continue with your experiment if you wish. I'm just warning you that unless you change your experimental design, no one will take your results seriously.

You also don't seem to have a clear, concrete end-goal. "Regenerating?" What does that mean? How will you quantify that? What criteria will you use to determine if a significant regeneration occured, especially since you don't have a control to compare to?
>>
>>719081080
You can remove exif data with a site like verexif()com. Just upload and hit "Remove Exif"
>>
>>719081482
I can imagine. That is no trivial task.

I have heard of a method similar to that, where a fluorophore that is not interacting (or bound) is a different color than when it is interacting. I forget what it is called off the top of my head.
>>
>>719081080
Google exif wipe. Run it through the app and voila. Also, unless u specifically have exif enabled on your camera photo settings, u don't save GPS location.
>>
>>719081551

I know the importance of a control group, since i study tissue and not the effect of hormone levels i only treat one side of the body at a time,

my end goal is to find a setting i read about that was later removed from a paper found while reading up on hearing damages, i didnt find it, but i remember the wavelenghts and modulation levels fairly well

if i succeed well? i might just play witch doctor and regenerate loss of hearing and call every modern doctor a fraud just for the trolling of it
>>
>>719081551
This

OP isn't doing science. He is exposing himself to undetermined amounts of energy and wavelength in an imprecise way, with no clear goal, and then attributing whatever happens afterwards to it.

The entire point of a control is to do a side by side experiment with and without the experimental element. That way you can attribute the results to what you did in the experiment and not something else.

Controls are key.
>>
>>719082013
>only treat one side of the body at a time

You have no fucking clue what you are doing
>>
>>719076018
What is the exact "treatment". Step by step pls.
>>
>>719082013
You think there was a reason that this result was removed from the paper?

Also, light therapy is not a new thing. It's used in medicine to treat hyperbillirubinemia in neonates. The light reacts with the bilirubin to make it more soluble in water, and thus can be excreted in urine.

Treating one side of the body at a time is not a proper control, because there may be systemic effects you are not accounting for which may affect the other side (assuming this is doing what you are expecting to at all.

I think you'd be better off doing this in mice first. You are jumping to humans without a mouse model for no discernible reason. You have not thought this experiment out to the extent that is needed.
>>
>>719082726
>You have not thought this experiment out to the extent that is needed.

He hasn't thought it out at all. Lots of people think they are scientists. Real science is fucking hard because you have to be almost OCD with how meticulous you are with your test conditions and controls.
>>
>>719082083
>>719082224
>>719070538
>>
>>719082890
Classic case of the Dunning-Kruger Effect.
>>
>>719082726

that i do

i wouldn't have started doing this unless i knew how to build high power modulators, (basically a series of cmos converters with a 4 mhz quartz cystal with bijunction transistors powering the mosfets going to the LED or laser tube) what ever... simplified

My newest version features a pic16f84a that gets its modulation patters from a .net application i wrote (easiest way to interface the com from a computer)

i figured it was already published and it wouldnt be rocket science to mimic it

im just unhappy i didnt save it the first time i saw it
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