Archived threads in /sci/ - Science & Math - 1245. page

http://bitesizebio.com/24002/problems-amplifying-gc-rich-regions-problem-solved/

>>8463934
Thanks mate. I actually just read this earlier. However, I have found very little information regarding the design of primers for a sequence so small. Any input would be immensely helpful.

>I know the standard is usually 18-22 bp
in cases like this, fuck the standard

what matters is GC, Tm, and specificity. If you can find a shorter primer that has a reasonable GC content and Tm but is still specific to your sequence, there's no reason not to at least try it. Primers are cheap.

in this case though, what I'd do is just try to change up my project so I avoided that problem sequence. You'll waste more time trying to optimize this trouble primer and the PCR reaction than you would just tossing a little work out and avoiding the problem in the first place.

PetroE
Bachelors, $100k starting

>>8463827
What about nuclear engineering or mathematics?

>>8463827
lol, no. i go to the best Petro E school in the country and our placement rate is abysmally low. those 100k + starting salaries ended when oil tanked to 40$ a barrel.

>could of

> requires unique ""trick""
how do you know its unique?

>could of

brainlet confirmed

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>>8463693
Because undergrad CS is a horrific waste of time. Just self study it.

>>8463704

You could make a list like this for literally any program of study.

>Implying CS = programming

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>>8463822
>>Implying CS = programming
>Too stupid to read the image

CS is a joke

Because closure is a useful property.

I like to think of the number line like a time line, minus is past, 0 is present and regular numbers are the future. The past goes on and it obviously exists. Also there is the concept of debt. You can owe someone cash but you wouldn't say I have 0 dollars when in debt, you would say I have negative x dollars. As soon as you get x amount you have 0 dollars, where as if you begin with 0 dollars and receive x amount...you have x amount of dollars.

>>8462502
You owe me ten dollars and at this moment you are broke.

You have -10 dollars in your account.

This is a complaint for your school's math department, not /sci/

>>8462458
Maybe you just suck at determining what is trivial and what is you being a lazy fuck

>>8462458
Cite what theorem you're using, don't just use a result implicitly in your proof.

Damaging. Needs more math. Normies think science is shit you discuss between bong hits.

>>8461609

>>8461604
Helping. A lot of redditors think they 'just totally get it, man' and go study math or physics to then only pay the big bucks a couple of the first semestees (the ones that are cheapest for the uni to keep but cost just as much as the future semesters for the students) and give money to our programs and then they just leave, giving our departments huge profit margins that they can use to fund us.

At my uni the stop students get paid a monthly salary funded right from brainlet pockets.

wow! you must be really smart

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>>8458921
Here is my bookshelf you fucking faggot.

You are either really old or really stupid lol. Not only are your books infinitely more expensive than mine, they take more space and will cause you trouble sometime in the future when you need to move or have to re-order to accomodate for more useful furniture.

>The "real books" industry still going strong in 2016 selling only to retards.

I wonder if only retards buy books, or if buying books makes you a retard.

What does /sci/ think?

>>8458953
Books are an aesthetic element in a room, faggot.

Also, take your pedophile calculus book to >>>/a/.

>>8457938

if you look in genetics studies youll find that the truth is somewhere in the middle. ofcourse there is variation between people across geographical space but there is no scientific way to divide these into non-arbitrary racial groups.

>>8457938

https://scholar.google.co.uk/scholar?q=race+human+diversity+genetics&btnG=&hl=en&as_sdt=0%2C5

this search will probably have studies you will want to look for, using an unbiased search

>>8457938

i dont think theres any genes that show a persons ethnicity. it wouldnt make sense.

>>8463457
Yea sure, why not just also place them onto a metal pot and heat that with a stove-top burner, like one would normally boil water?

>>8463459
unfortunately I only have one pot and it's dirty. And I can't clean it because no water to clean with :(

>put ice cubes in a clean glass
>put glass on top of or near a heat source (body, PC, heater, lamp)
>drinkable cold water

>>8463345
short reviews across a wide variety of topics, a few deal with set theory
https://www.ocf.berkeley.edu/~abhishek/chicmath.htm

the above link may not provide you with what you are looking for right now, still worth looking at

best of luck

>>8463345
What material did you use to learn the high school math? I don't study formally right now because wife is in college but I've been looking for good resources to relearn middle and high school math, and maybe science.

Good luck on your search.

>>8463354
Thanks!

>>8463308
Use (a + b)^n =(n choose k)a^kb^(n-k)

>>8463311
Not sure I see it. Wouldn't it need to be 2ab for binomial theorem?

>>8463308
I'd stick with multiplying

>it's actually a theorem.

>define """""large"""""

>>8463298
Isn't it technically "the sum of a large number of uniform random variables will tend toward a normal random distribution"?

>>8463271
it unfortunately doesn't matter as long as there's first past the post voting.

Also I think there is a bias you have to worry about

>>8463271
I did some months ago. I remember that I sided as much with Trump as I did with Hillary and Bernie was the guy I sided with the most.

That felt sad. The media would give you the impression that Trump is the anti Hillary and Hillary is the anti trump but aside from a couple meme issues like 'muh wiimmenzz' they are pretty much equivalent.

Proof of how the supposed left is just the center right. Democrats are just republicans who care a little more about personal rights and thus support stuff like gay marriage, unlike republicans who see personal rights as non issues.

>>8463274
What bias do you mean? The one from the site or from me kind of not wanting to align with Hillary?

And what do you think about the idea to replace elections with a test like this, and candidates with ideas?

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Fivehead; I guess from a distance it doesn't look horrible.

still kind of deep. flash with cold water to neutralize ph balance. don't put anything on it in case of mixing with the bleach.

then is up to vitamins and exercise to push the skin cells up,flake off the old ones, and get rid of the scar.

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>>8462981


-poor cold water on it
-neutralize with solution with basic ph, like baking soda in water
-put creams for burns, like pic
-put cream/ointment to moisturize, like hydromol or some biafine
-when going out, put sun screen, like spf 50