in this ITT: memes only you remember
>>4573680
smol doge
w-why are you making me wear girls clothes?
i'm a boy...
Protease is used in detergents to remove protein-based stains such as blood, mucus, feces, and various foods such as egg and gravy. These substances are almost insoluble and they tend to adhere to textiles and other surfaces. The protease hydrolysis products are peptides which are readily dissolved or dispersed in the washing liquor. All proteolytic enzymes described are fairly non-specific serine endoproteases, cleaving peptide chains preferentially at the carboxyl side of hydrophobic amino acid residues. The enzymes convert their substrates into small, readily soluble fragments which can be removed easily from fabrics. Only serine protease can be used in detergent formulations, as thiol proteases such as papain would be oxidized by the bleaching agents, acidic proteases are not active at common laundry conditions, and metalloproteases such as thermolysin would lose their metal cofactors because of complexation with the water-softening agents or hydroxyl ions. The most important enzyme in laundry detergents is subtilisin, which cleaves many peptide bonds, especially hydrophobic ones, and which is active in the desired alkaline pH range between 8 and 10.5, in which most washing cycles occur. The small, compact shape (MW 27.5 kDa; monomer) and the known 3D structure were additional reasons for developing this enzyme further.
A protease such as Esperase has a pH activity optimum and also a high temperature optimum of 60– 70 °C compatible with the high values of laundry cycle operation. This makes Esperase an effective means of improving the washing efficacy in industrial laundering systems with their relatively short washing time of 3–6 minutes. Industrial laundering systems often face special challenges such as cleaning of textiles heavily soiled with blood, e.g., from hospitals and slaughterhouses. In the development process at Genencor, subtilisin was found to be labile to oxidation: the cause was found to be a methionine side chain in position 222 (M222), directly adjacent to the catalytic serine (S221, part of the catalytic triad). Oxidation of the side-chain sulfur to the sulfoxide led to a 90% activity decrease. Genencor solved the problem by site-directed mutagenesis in position 222; substitution of methionine by all other 19 proteinogenic amino acids yielded positive results in the cases of leucine, serine, and especially alanine, in that the mutated enzyme was stable for the required one hour in a 1 m H2O2 solution. This accomplishment stands as the first great success of protein engineering.
In the farm animal business, speed of growth is essential for rapid turnaround of delivery to market. An adequate diet containing all the required nutrients is essential not just for rapid growth but also for the prevention of disease. Phosphorus, essential for DNA synthesis and energy metabolism, but mainly also for proper skeletal growth, is supplied through plant components called phytates which are hexaphosphorylated inositols (or phosphorylated cyclic sugar alcohols); the acid is called phytic acid. As much as 80% of the phosphorus in cereal grains and oilseed meals is chemically bound in the form of phytate. This phosphorus has a very low bioavailability; pigs and chickens digest it poorly because they lack the enzyme phytase. All of this unavailable phosphorus is excreted in the feces. The US poultry industry, which contributes more than $ 12 billion annually to the economy, includes some 75 000 growers nationwide who produce more than 6.5 billion broilers, 285 million turkeys, and 20 million ducks. These operations generate 20 million tons of manure annually, which must be disposed of in an environmentally safe manner. Generally, the waste is applied to the land as a fertilizer. At the same time, however, producers must add inorganic phosphorus to the animals’ rations to meet their needs – and part of this supplemental phosphorus is also excreted. This leads to a vast oversupply of the total amount of phosphorus in the animals’ diet, causing a waste problem that places a strain on waters such as rivers and creeks in the vicinity of animal farms but ultimately also aquifers. Excess phosphorus in waterways can cause rapid algal growth and can affect the amount of oxygen in the water, leading to fish kills and other severe environmental, health, and economic problems.
Who else thinks 80's Cyndi Lauper was kinda hot?
>>4573618
i brefer ket bosh
>>4573626
monery
What would you do if you saw this while you were waking down the street [s4s]?
>>4573552
am moch more atract to mayby a bon or guzelle
(i giv her something to guzelle heh)
cringe because I used to obsess over that movie
>>4573552
i'd build a time machine and tell ur mom to abort you
Trips or Godly luck choose what thread is about.
Your fortune: Good news will come to you by mail
>>4573495
I just did, lol.
This thread is now about trips or godly luck choosing the next topic.
Your fortune: Good news will come to you by mail
danke maymays :^)
Your fortune: Godly Luck
nice shot for a rookie kiddo ;^)
>>4573484
please delete this
children might see
>>4573484
I scrolled past this thumbnail without realizing what it is several times. Don't post this shit here.
Spiky moslem
>>4573476
hedgehoge :)
>>4573476
dang.......
that moslem ladey sexey
i want to pentrate her
As salamu alaikum wa rahmatullah wa barakatuhu
just a few thingds. sometimes when i post a post on the websight here i go to post another post and i get a countdown. it telss me how long i need to wait for the next post. but sometimes i dont get the count down and i try to count the time on my watch or just count out loud and some times i miss it and i get pic realted. how come some times i get the nice timer and other times i just dont. do you have the problem is this because of my internet at my house or is it just for some like a PC or a mac but i do not use a mac. it also does not timer on my ohone when i post from like the restoroom or from some where else. so then i have to count too. also some times when i turn on my coputer i have the blue light that shows the that the coputer is "on" but some times it does it will turn on but the light does not turn on (but the coputer goes on). why is that? i dont idk if the light is just loose? or dose it mean it has some probleam. dose this have anything to do with the timer. should i deleter my intertnet history or just leave it? i got told that if i left it then the internet would be faster because it dfosent have to work as hard to download all of the pictures again, is that true? also when i start my coputer some times it makes more then one beeper and i think its wrong but it seems fine. when some times when i have the coputer on it gets slow and thing pop up. is there a probleam oir it it fine? also how much ram should i have? is a hard drive good for the coputer or should i just use alot of ram. also what kind of mac or pc "cpu" should i up grade ot if i pay a man to up grade it because it should be faster? will it make it faster to get a bigger ram or just a hard drive? last thing some times when i use this web sight the pictures come up but when i go to click they come up more and the whole thing "jumps" down and then i when i go to click the word it "jump" down about an inch is this normal? but if i wait some times it just goes in and i can click it fine?
>I am seated in an office, surrounded by heads and bodies. My posture is consciously congruent to the shape of my hard chair. This is a cold room in University Administration, wood-walled, Remington-hung, double-windowed against the November heat, insulated from Administrative sounds by the reception area outside, at which Uncle Charles, Mr. deLint and I were lately received.
I am in here.
Three faces have resolved into place above summer-weight sportcoats and half-Windsors across a polished pine conference table shiny with the spidered light of an Arizona noon. These are three Deans - of Admissions, Academic Affairs, Athletic Affairs. I do not know which face belongs to whom.
I believe I appear neutral, maybe even pleasant, though I've been coached to err on the side of neutrality and not attempt what would feel to me like a pleasant expression or smile.
I have committed to crossing my legs I hope carefully, ankle on knee, hands together in the lap of my slacks. My fingers are mated into a mirrored series of what manifests, to me, as the letter X. The interview room's other personnel include: the University's Director of Composition, its varsity tennis coach, and Academy prorector Mr. A. deLint. C.T. is beside me; the others sit, stand and stand, respectively, at the periphery of my focus. The tennis coach jingles pocket-change. There is something vaguely digestive about the room's odor.
The Dean at left, a lean yellowish man whose fixed smile nevertheless has the impermanent quality of something stamped into uncooperative material, is a personality-type I've come lately to appreciate, the type who delays need of any response from me by relating my side of the story for me, to me. Passed a packet of computer sheets by the shaggy lion of a Dean at center, he is peaking more or less to these pages, smiling down.
(Continues...)
has a man ever been so dick-lickingly willing to spurt up a rudimentary statistic from his ragged damaged mouthhole for nothing but the emotional reactions & ensuing default-mode votes of the mal-educated hordes? buttugly blubbering hunchback bernie knows what he's doing, and it's textbook deceit. a woman's career choice is not sexism. women exercising their last vestiges of primordial knowledge & evolutionarily-ingrained tendencies toward the home & family is not discrimination. it's the fuck what drives them. it's how they managed to raise the great thinkers of yestercentury. 'no more of that for you, young lady! instead of giving young minds an incubatory dreamworld to fascinate themselves with, instead of being the cohesion of compassion that used to keep families in tact, you're missus worker woman! you can do anything a man can!' cut to some spiritually-adrift chick slumped over a standing desk at some godforsaken afterhour, sporting a pair of virtue-signaling relaxed-fit denim jeans, planning a thirtieth-birthday suicide, loathing the last bleak reason for being that she supplanted her real dreams for. that's how your fuckbackwards social justice is served, sandy. you've been debunked, berny boy. you've been debunked a thousand times over. when you repeat this shit on your inglorious road-to-nowhere campaign trail, i hope you feel a twinge. just a little pin-prick at your heart, to remind you of what a shamelessly demagogic dolt you are. i piss in your senate seat, you senile ghoul.
>>4573409
rly good book 1 of my favrites evn tho meme
this board is so slow
ok
That is because we are developing epic memes 24/7/365/2016!
Us mEmErs cannot be everywhere at the same time, you know.
Your fortune: Good Luck
no, this is nice board
Your fortune: Better not tell you now
hahaha... so fucken disgusting my god.
>>4573265
dumbshit let your cat on the table you are truly filthy. big fat sack of shit, i shrivel ur ovary bisthc
>>4573289
bumbing cause im gna replie in depth l8r
snooze bommpe
>>4573289
Post one from the Marylin Monroe folder plox.l
how's your dental work
>>4573220
nice quads
>>4573220
Sponges don't have teeth?!
poopgril
there's something very wrong with you
>>4573205
popgil is o.k. but she is below me, in all honesty. she found a fukkup thats willing to take whatever quantum of solace he can muster up for a skype-based relationship. i simply match-made them, I am responsible for their MUTUAL albeit transient happiness. i so loved popgil's style, that i got her to reveal a e-mail, and that is probley when dork lard jumped at the chance to get w/ that bubbly little mutt. that is all well and good. i enjoy nicer things, better ppl, i crawl like a viper through these suburban streets, i trawl the megahertz. pop gil is a home-body princess-point mommys-gril, neither her nor dorklard enjoys these luxuries. so they can have each othre. if i really gave a hoot I WOULD have emailed first, but i DIDNT. i like her POSTING SCHTAO and i think she sexey in special way but i am not dork-lard desperate! this is the critical distinction!
pic is popgils boney ass arm, who care not me
poopgril
1. Wayne Coyne threads (worse than doge)
2. pedo and loli threads
3. gay porn threads
4. bune raven (finally fading away after 6 miserable months)
Did I miss any
>>4573164
nohp
>>4573164
you missed your ugly mum's fat arse
>>4573173
calm down kid what are you from scotland why dont you buckle up your kilt and take your knee socks and bagpipe over to a rude board like /b/
.
>>4573137
my dick is hard
>>4573137
Dumb animeposter
Am africa man
>>4573111
Three.
>>4573122
nice twos